Empty and Full Separation of Adeno-Associated Virus Vectors by Anion Exchange Membrane Chromatography

Time: 10:00 am
day: Day One

Details:

  • Here we assess the performance of anion exchange (AEX) membrane chromatography as the polishing stage of an AAV platform process following affinity purification. – – Utilizing a novel 1mS/cm step gradient approach, we are able to demonstrate separation of empty and full AAV capsids of serotypes 5, 8, and 9 with the Mustang Q membrane. This process maximizes the high flow rate benefits of membrane chromatography relative to traditional column chromatography, while providing improved separation
  • Distinct populations in the UV 260/280 chromatogram, analytical trends with PCR and ELISA, and capsid standards prepared by ultracentrifugation reaffirm separation
  • This technique is scalable between the 0.86 mL Mustang Q XT Acrodisc and 5 mL XT capsule, and effectively clears residual host cell protein and DNA contaminants

Speakers: